Nonoxidative fungicidal mechanisms of mammalian granulocytes: demonstration of components with candidacidal activity in human, rabbit, and guinea pig leukocytes.

نویسندگان

  • R I Lehrer
  • K M Ladra
  • R B Hake
چکیده

Granulocytes from the peripheral blood of normal subjects and a patient with hereditary myeloperoxidase deficiency were homogenized in 0.34 M sucrose. A granule-rich fraction, prepared by sedimentation at 27,000 x g for 20 min, contained components that killed C. parapsilosis in vitro. These were extractable with 0.01 M citric acid and were shown by micropreparative polyacrylamide electrophoresis to be multiple. The candidacidal activity of these neutrophil components was heat stable and they were somewhat more active at pH 5.0 than at pH 7.0. When rabbit or guinea pig heterophils were obtained from sterile peritoneal exudates and similarly fractionated, they also were found to contain components that killed C. parapsilosis in vitro. These were primarily associated with a group of lysosomal cationic proteins lacking direct counterpart in human neutrophils. Among the candidacidal components of the human neutrophil was a protein, more cationic than lysozyme, that exhibited naphthol-ASD acetate esterase activity.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A Survey on the Gastrointestinal Parasites of Rabbit and Guinea Pig in a Laboratory Animal House

There is documented evidence that infection in laboratory animals can often influence the outcome of experiments. All infections, apparent or inapparent, are likely to increase biological variability. As a research project concerning the diversity and distribution of parasites of rabbit and guinea pig in a conventional laboratory animal house, about 87 rabbits (from 700 ) and 105 guinea pigs (f...

متن کامل

STUDIES ON THE TISSUE SPECIFICITY OF COLONY STIMULATING FACTOR

In this study granulocyte-macrophage colony stimulating factor (CSF) was prepared from rabbit and guinea pig lung tissues. The biological activity of these CSFs was measured on semisolid agar culture and compared with those of mouse and rat. Weight of animals, incubation time and cross-reactivity of CSFs with different bone marrow sources were also investigated. The results showed that both...

متن کامل

Studies on the antispasmodic and cardiovascular activities of methanolic crude extract of Andropogon muricatus. Nash

Andropogon muricatus Nash., has been used in the traditional medicine as an antispasmodic and in palpitation and this study was aimed to provide scientific basis for its use in gastrointestinal and cardiovascular disorders. Apporaximatly 2 cm long segments of rabbit jejunum and 2-3 mm wide aortic rings and strips of guinea-pig atria were incubated in normal Tyrod’s and Krab’s solutions at 37°C ...

متن کامل

Studies on the antispasmodic and cardiovascular activities of methanolic crude extract of Andropogon muricatus. Nash

Andropogon muricatus Nash., has been used in the traditional medicine as an antispasmodic and in palpitation and this study was aimed to provide scientific basis for its use in gastrointestinal and cardiovascular disorders. Apporaximatly 2 cm long segments of rabbit jejunum and 2-3 mm wide aortic rings and strips of guinea-pig atria were incubated in normal Tyrod’s and Krab’s solutions at 37°C ...

متن کامل

Development of a Sensitive Spectrofluorometric-Multivariate Calibration Method for Enzyme Kinetic of Aldehyde Oxidase

Attempts to obtain experimental values for the kinetic parameters of phenanthridine oxidation by guinea pig or rabbit liver aldehyde oxidase using common spectrophotometric methods have not been successful due to a lower limit of detection. In the present study, a new spectrofluorimetric assay in combination with a multivariate calibration method for enzymatic kinetic study of aldehyde oxidase ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Infection and immunity

دوره 11 6  شماره 

صفحات  -

تاریخ انتشار 1975